Wounds may be acute, subacute, or chronic and are the common clinical life entities. It is a complex biochemical process to restore the cell structures that depends upon cell proliferation and migration, mainly by keratinocytes and fibroblast cells. The aim of the present study was to evaluate the effect of the Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HaCaT cell line (Human keratinocytes) and DMEM (Dulbecco’s Modified Eagle Medium) for wound healing action using scratch assay (48 hours) against positive control i.e., recombinant Human Epidermal Growth Factor (Hu-EGF, 30 ng/mL). In vitro scratch assay monitored proliferation and migration of keratinocytes in the scratched wounded area using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEM group have significantly higher percentage of keratinocytes migration i.e., 35.4%, while no migration was monitored in the Biofield Energy Treated HaCaT cells as compared with the baseline control group. Additionally, the percentage of scratch area was significantly decreased by 7.3% in the Biofield Energy Treated DMEM, while an increased scratched area was reported by 14% in the Biofield Energy Treated cells. Hence, the results using scratch assay showed a promising scientific approach to differentiate the Biofield Energy Treatment on cells or media for their wound healing potential. The data concluded that The Trivedi Effect® has the significant capability in wound healing activity in DMEM as compared with the HaCaT cell line with respect to the cell migration and reduced wound scratched area. Consciousness Energy Healing Treatment (The Trivedi Effect®) can be used as a complementary and alternative approach to improve the cellular migration and proliferation and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.
Authors are grateful to Dabur Research Foundation, Trivedi Global, Inc., Trivedi Science, Trivedi Testimonials and Trivedi Master Wellness for their support throughout the work.
Alonso JE, Lee J, Burgess AR, Browner BD (1996) The management of complex orthopaedic injuries. Surg Clin North Am. 76: 879-903.
Becker RO (1960) A description of the integrated system of direct currents in the salamander. IRE Trans Biomed Electron 7: 202.
Becker RO (1961) The bioelectric factors in amphibian limb regeneration. J Bone Joint Surg. 43A: 643.
Becker RO, Selden G (1985) The body electric: Electromagnetism and the foundation of life, New York City, William Morrow and Company.
Dorai AA (2012) Wound care with traditional, complementary and alternative medicine. Indian J Plast Surg 45: 418-424.
Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
Funk RH, Monsees T, Ozkucur N (2009) Electromagnetic effects—from cell biology to medicine. Prog Histochem Cytochem 43: 177-264.
Giasson M, Bouchard L (1998) Effect of therapeutic touch on the well-being of persons with terminal cancer. J Holist Nurs 16: 383-398.
Goodwin PK, Travis TE, Jordan MH, Flanagan KE, Pavlovich AR, Shupp JW (2014) Prospective evaluation of biofield energy therapy for burdensome symptoms of burn injury: A pilot study. J Pain Relief 3: 1-5.
Guo S, DiPietro LA (2010) Factors affecting wound healing. J Dental Res. 89: 219-229.
Gurtner GC, Werner S, Barrandon Y, Longaker MT (2008) Wound repair and regeneration. Nature 453: 314-321.
Hines ME, Wardell DW, Engerbretson J, Zahourek R, Smith MC (2015) Holistic nurses’ stories of healing of another. J Holist Nurs 33: 27-45.
Hosoi J, Abe E, Suda T, Kuroki T (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 45: 1474-1478.
Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
Natarajan S, Williamson D, Stiltz AJ, Harding K (2000) Advances in wound care and healing technology. Am J Clin Dermatol. 1: 269-275.
Peck SD (1998) The efficacy of therapeutic touch for improving functional ability in elders with degenerative arthritis. Nurs Sci Q 11: 123-132.
Reuter J, Merfort I, Seelinger G, Wölfle U, Schempp CM (2009) Botanicals in dermatology and skin health. In: Cooper, R., Kronenberg, F. (Eds.), Botanical medicine. From bench to beside. Mary Ann Liebert Inc., New Rochelle, NY. USA.
Schafer M, Werner S (2007) Transcriptional control of wound repair. Annu Rev Cell Dev Biol 23: 69-92.
Schlitz M, Hopf HW, Eskenazi L, Vieten C, Radin D (2012) Distant healing of surgical wounds: an exploratory study. Explore (NY) 8: 223-230.
Schmidt C, Fronza M, Goettert F, Geller F, Luik S, Flores EMM, Bittencourt CF, Heinzmann BM, Laufer S, Merfort I (2009) Biological studies on Brazilian plants used in wound healing. J Ethnopharmacol. 122: 523-532.
Seeliger C, Falldorf K, Sachtleben J, Griensven M van (2014) Low-frequency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts. Eur J Med Res. 19: 37.
Tabish SA (2008) Complementary and Alternative Healthcare: Is it Evidence-based? Int J Health Sci (Qassim) 2: V-IX.
Tao H, Berno AJ, Cox DR, Frazer KA (2007) In vitro human keratinocyte migration rate are associated with SNPs in the KRT1 interval. PLoS ONE 2: e697.
Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015l) Spectroscopic characterization of disulfiram and nicotinic acid after biofield treatment. J Anal Bioanal Tech 6: 265.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015c) Antibiogram and genotypic analysis using 16S rDNA after biofield treatment on Morganella morganii. Adv Tech Biol Med 3: 137.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015d) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015e) Morphological and molecular analysis using RAPD in biofield treated sponge and bitter gourd. American Journal of Agriculture and Forestry 3: 264-270.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015f) Effect of biofield energy treatment on chlorophyll content, pathological study, and molecular analysis of cashew plant (Anacardium occidentale L.). Journal of Plant Sciences 3: 372-382.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015b) Antibiogram of biofield-treated Shigella boydii: Global burden of infections. Science Journal of Clinical Medicine 4: 121-126.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015g) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015h) Effect of biofield treated energized water on the growth and health status in chicken (Gallus gallus domesticus). Poult Fish Wildl Sci 3: 140.
Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015i) Characterization of physical, thermal and spectroscopic properties of biofield energy treated p-phenylenediamine and p-toluidine. J Environ Anal Toxicol 5: 329.
Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015j) Evaluation of physical, thermal and spectroscopic properties of biofield treated p-hydroxyacetophenone. Nat Prod Chem Res 3: 190.
Trivedi MK, Nayak G, Tallapragada RM, Patil S, Latiyal O, Jana S (2015n) Effect of biofield treatment on structural and morphological properties of silicon carbide. J Powder Metall Min 4: 132.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015k) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6: 395.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015a) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015m) Characterization of physical, thermal and structural properties of chromium (VI) oxide powder: Impact of biofield treatment. J Powder Metall Min 4: 128.
Turner JG, Clark AJ, Gauthier DK, Williams M (1998) The effect of therapeutic touch on pain and anxiety in burn patients. J Adv Nurs 28: 10-20.
United States National Institutes of Health, National Center for Complementary and Alternative Medicine (2007). CAM at the NIH: Focus on complementary and alternative medicine (newsletter). Volume XIV, No. 2, New Integrative Medicine Consult Service Established.
Velnar T, Bailey T, Smrkolj V (2009) The wound healing process: An overview of the cellular and molecular mechanisms. J Int Med Res. 37: 1528-1542.
Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, Arauz R, Rachlin K (2013) Evaluation of biofield treatment dose and distance in a model of cancer cell death. J Altern Complement Med 19: 124-127.
Cite this work
Researchers should cite this work as follows: