An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen

By Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Mayank Gangwar2, Snehasis Jana2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

Published on


Stenotrophomonas maltophilia (S. maltophilia) is a Gram-negative bacillus, an opportunistic pathogen, particularly among nosocomial infections. Multi-drug resistant strains are associated with very high rate of morbidity and mortality in severely immunocompromised patients. Present study was designed to evaluate the effect of biofield treatment against multidrug resistant S. maltophilia. Clinical sample of S. maltophilia was collected and divided into two groups i.e. control and biofield treated which were analyzed after 10 days with respect to control. The following parameters viz. susceptibility pattern, minimum inhibitory concentration (MIC), biochemical studies and biotype number of both control and treated samples were measured by MicroScan Walk-Away® system. The results showed an overall change of 37.5% in susceptibility pattern and 39.4% in biochemical study while 33.3% changes in MIC values of tested antimicrobials after biofield treatment. Further, the treated group of S. maltophilia has also shown a significant change in biochemical reactions followed by its biotype number as compared to control group. Biochemical reactions of treated group showed negative reaction to acetamide and positive reactions to colistin, glucose, adonitol, melibiose, arabinose, nitrate, oxidation-fermentation, raffinose, rhaminose, sorbitol, sucrose, and Voges-Proskauer as compared with control. The biofield treatment showed an alteration in MIC values of amikacin, amoxicillin/K-clavulanate, chloramphenicol, gatifloxacin, levofloxacin, moxifloxacin, ceftazidime, cefotetan, ticarcillin/K-clavulanate, trimethoprim/sulfamethoxazole. Altogether, data suggest that biofield treatment has significant effect to alter the sensitivity pattern of antimicrobials and biotype number against multidrug resistant strain of S. maltophilia.


1. Singh V (2013) Antimicrobial resistance. In: Microbial pathogens and strategies for combating them: science, technology and education. Formatex Research Center, India.

2. Popęda M, Płuciennik E, Bednarek AK (2014) [Proteins in cancer multidrug resistance]. Postepy Hig Med Dosw (Online) 68: 616-632.

3. Livermore DM (2004) The need for new antibiotics. Clin Microbiol Infect 10 Suppl 4: 1-9.

4. Jumaa PA, Sonnevend A, Pàl T, El Hag M, Amith R, et al. (2006) The molecular epidemiology of Stenotrophomonas maltophilia bacteraemia in a tertiary referral hospital in the United Arab Emirates 2000-2004. Ann Clin Microbiol Antimicrob 5: 32.

5. Jang TN, Wang FD, Wang LS, Liu CY, Liu IM (1992) Xanthomonas maltophilia bacteremia: an analysis of 32 cases. J Formos Med Assoc 91: 1170-1176.

6. Victor MA, Arpi M, Bruun B, Jønsson V, Hansen MM (1994) Xanthomonas maltophilia bacteremia in immunocompromised hematological patients. Scand J Infect Dis 26: 163-170.

7. Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL (2015) Trends in the use of complementary health approaches among adults: United States, 2002-2012. Natl Health Stat Report : 1-16.

8. Lucchetti G, de Oliveira RF, Gonçalves JP, Ueda SM, Mimica LM, et al. (2013) Effect of Spiritist "passe" (Spiritual healing) on growth of bacterial cultures. Complement Ther Med 21: 627-632.

9. Maxwell J (1865) A dynamical theory of the electromagnetic field. Phil Trans R Soc Lond 155: 459-512.

10. Movaffaghi Z, Farsi M (2009) Biofield therapies: biophysical basis and biological regulations? Complement Ther Clin Pract 15: 35-37.

11. Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of pogostemon cablin (Benth.). Biotechnology 11: 154-162.

12. Altekar N, Nayak G (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.

13. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mat Sci 32: 471-479.

14. Trivedi MK, Patil S, Tallapragada RM (2012) Thought intervention through biofield changing metal powder characteristics experiments on powder characterisation at a PM Plant. Lecture Notes in Electrical Engineering 173: 247-252.

15. Trivedi MK, Patil S (2008) Impact of an external energy on Staphylococcus epidermis [ATCC-13518] in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 4: 230-235.

16. Trivedi MK, Patil S (2008) Impact of an external energy on Yersinia enterocolitica [ATCC-23715] in relation to antibiotic susceptibility and biochemical reactions: an experimental study. Internet J Alternat Med 6: 13.

17. Trivedi MK, Bhardwaj Y, Patil S, Shettigar H, Bulbule A (2009) Impact of an external energy on Enterococcus faecalis [ATCC-51299] in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 5: 119-130.

18. Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial Susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.

19. Krucoff MW, Crater SW, Gallup D, Blankenship JC, Cuffe M, et al. (2005) Music, imagery, touch, and prayer as adjuncts to interventional cardiac care: the monitoring and actualisation of noetic trainings (MANTRA) II randomized study. Lancet 366: 211-217.

20. Tenover FC (2006) Mechanisms of antimicrobial resistance in bacteria. Am J Med 119: S3-10.

21. He X, Li S, Kaminskyj SG (2013) Using Aspergillus nidulans to identify antifungal drug resistance mutations. Eukaryot Cell 13: 288-294.

22. Alekshun MN, Levy SB (2007) Molecular mechanisms of antibacterial multidrug resistance. Cell 128: 1037-1050.

23. Entenza JM, Moreillon P (2009) Tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies. Int J Antimicrob Agents 34: e1-e9.

24. Maisetta G, Mangoni ML, Esin S, Pichierri G, Capria AL, et al. (2009) In vitro bactericidal activity of the N-terminal fragment of the frog peptide esculentin-1b (Esc 1-18) in combination with conventional antibiotics against Stenotrophomonas maltophilia. Peptides 30: 1622-1626.

25. Zelenitsky SA, Iacovides H, Ariano RE, Harding GK (2005) Antibiotic combinations significantly more active than monotherapy in an in vitro infection model of Stenotrophomonas maltophilia. Diagn Microbiol Infect Dis 51: 39-43.

26. Carmody LA, Spilker T, LiPuma JJ (2011) Reassessment of Stenotrophomonas maltophilia phenotype. J Clin Microbiol 49: 1101-1103.

27. Turner JG, Clark AJ, Gauthier DK, Williams M (1998) The effect of therapeutic touch on pain and anxiety in burn patients. J Adv Nurs 28: 10-20.

28. Koithan M (2009) Introducing Complementary and Alternative Therapies. J Nurse Pract 5: 18-20.

29. Hintz KJ, Yount GL, Kadar I, Schwartz G, Hammerschlag R, et al. (2003) Bioenergy definitions and research guidelines. Altern Ther Health Med 9: A13-30.

Cite this work

Researchers should cite this work as follows:

  • Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia : An Emerging Global Opportunistic Pathogen. Clin Microbiol 4: 211. doi: 10.4172/2327-5073.1000211

  • Mahendra Kumar Trivedi; Shrikant Patil; Harish Shettigar; Mayank Gangwar; Snehasis Jana (2019), "An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen,"

    BibTex | EndNote