Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone

By Mahendra Kumar Trivedi1, Gopal Nayak1, Shrikant Patil1, Rama Mohan Tallapragada1, Snehasis Jana2, Rakesh Kumar Mishra2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

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Abstract

In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.

References

1. Elzoghby AO, Abo El-Fotoh WS, Elgindy NA (2011) Casein-based formulations as promising controlled release drug delivery systems. Journal of Controlled Release 153: 206-216.

2. Lewis DH (1990) Biodegradable Polymers as Drug Delivery Systems. New York: Marcel Dekker. 75: 1-18

3. Bryant CM, McClement DJ (1998) Molecular basis of protein functionality with special consideration of cold-set gels derived from heat-denatured whey. Trends in Food Science & Technology 9: 143-151.

4. Clark AH (1998) Gelation of globular proteins: Functional Properties of Food Macromolecules. Gaithersburg MD: Aspen.

5. Dickinson E. (2003) Colloidal aggregation: mechanism and implications: Food Colloids, Biopolymers and Materials. Cambridge: Royal Society of Chemistry.

6. Walstra P (2003) Studying food colloids: past, present and future: Food Colloids, Biopolymers and Materials. Cambridge: Royal Society of Chemistry.

7. Chen L, Remondetto GE, Subirade M (2006) Food protein-based materials as nutraceutical delivery systems. Trends in Food Science & Technology 17: 272-283.

8. Chen L, Subirade M (2008) Food-protein-derived materials and their use as carriers and delivery systems for active food components. Delivery and Controlled Release of Bioactives in Foods and Nutraceuticals. UK: Woodhead Publishing Ltd.

9. Panyam KD, Kilara Arun (2003) Peptides from milk proteins and their properties. Critical Reviews in Food Science and Nutrition 43: 607-633.

10. Korhonen H, Pihlanto A (2003) Bioactive peptides: new challenges and opportunities for dairy industry. Australian Journal of Dairy Technology 58: 129-134.

11. Livney YD (2010) Milk proteins as vehicles for bioactives. Current Opinion in Colloid & Interface Science 15: 73-83.

12. Wang J, Su Y, Jia F, Jin H (2013) Characterization of casein hydrolysates derived from enzymatic hydrolysis. Chemistry Central Journal 7: 62.

13. Myers R (2003) The basics of chemistry. Westport, Connecticut, London: Greenwood Press.

14. Trivedi MK, Tallapragada RR (2008) A transcendental to changing metal powder characteristics. Metal Powder Report 63: 22- 28 31.

15. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bulletin of Materials Science 32:471-479.

16. Trivedi MK, Tallapragada RR (2009) Effect of super consciousness external energy on atomic, crystalline and powder characteristics of carbon allotrope powders. Materials Research Innovations 13- 473-480.

17. Trivedi MK, Patil S, Tallapragada RM (2012) Thought Intervention through Biofield Changing Metal Powder Characteristics Experiments on Powder Characterisation at a PM Plant , Future Control and Automation Lecture Notes in Electrical Engineering Volume 17: 247-252 .

18. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of Biofield Treatment on the Physical and Thermal Characteristics of Vanadium Pentoxide Powders. Journal of Material Sciences & Engineering S11:001.

19. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of Silicon, Tin and Lead powders. Journal of Material Sciences & Engineering 2:125.

20. Trivedi MK, Patil S, Tallapragada RM (2014) Atomic, Crystalline and Powder Characteristics of Treated Zirconia and Silica Powders. J Material Sci Eng 3: 144.

21. Trivedi MK, Patil S, Tallapragada RMR (2015) Effect of Biofield Treatment on the Physical and Thermal Characteristics of Aluminium Powders. Industrial Engineering & Management 4:151.

22. Shinde V, Sances F, Patil S, Spence A (2012) Impact of Biofield Treatment on Growth and Yield of Lettuce and Tomato. Australian Journal of Basic and Applied Sciences 6: 100-105

23. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of Biofield Treatment on Ginseng and Organic Blueberry Yield. AGRIVITA, Journal of Agricultural Science 35: 1991

24. Lenssen AW (2013) Biofield and Fungicide Seed Treatment Influences on Soybean Productivity, Seed Quality and Weed Community. Agricultural Journal 8: 138-143.

25. Altekar N, Nayak G (2015) Effect of Biofield Treatment on Plant Growth and Adaptation. Journal of Environment and Health sciences 1: 1-9

26. Trivedi M, Patil S (2008) Impact of an external energy on Staphylococcus epidermis [ATCC –13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study. Journal of Accord Integrative Medicine 4: 230-235.

27. Trivedi M, Patil S (2008) Impact of an external energy on Yersinia enterocolitica [ATCC –23715] in relation to antibiotic susceptibility and biochemical reactions: An experimental study. The Internet Journal of Alternative Medicine 6.

28. Trivedi M, Bhardwaj Y, Patil S, Shettigar H, Bulbule A (2009) Impact of an external energy on Enterococcus faecalis [ATCC – 51299] in relation to antibiotic susceptibility and biochemical reactions - An experimental study. Journal of Accord Integrative Medicine 5: 119-130.

29. Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of Biofield Treatment on Growth and Anatomical Characteristics of Pogostemon cablin (Benth.). Biotechnology 11:154-162

30. Colthup NB, Daly LH, Wiberley SE (1975) Introduction to Infrared and Raman Spectroscopy. New York: Academic Press.

31. Barth A (2007) Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1767: 1073-1101.

32. Torii H, Tatsumi T, Tasumi M (1998). Effects of hydration on the structure, vibrational wavenumbers, vibrational force field and resonance Raman intensities of N-methylacetamide. Journal of Raman Spectroscopy 29: 537-546.

33. Mennucci B, Martinez JM (2005) How to model solvation of peptides? Insights from a quantum-mechanical and molecular dynamics study of N-methylacetamide. I. Geometries, infrared, and ultraviolet spectra in water. The Journal of Physical Chemistry B 109: 9818-9829.

34. Bendz D, Tuchsen PL, Christensen TH (2007) The dissolution kinetics of major elements in municipal solid waste incineration bottom ash particles. Journal of Contaminant Hydrology 94: 178-194.

35. Chandler AJ, Eighmy TT, Hartlen J, Hjelmer O, Kosson DS, et al. (1997) Municipal solid waste combustion residues: the international ash working group: Studies in Environmental Science. Amsterdam: Elsevier science.

Cite this work

Researchers should cite this work as follows:

  • Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, et al (2015) Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone. Clin Pharmacol Biopharm 4: 138. doi:10.4172/2167-065X.1000138
     

  • Mahendra Kumar Trivedi; Gopal Nayak; Shrikant Patil; Rama Mohan Tallapragada; Snehasis Jana; Rakesh Kumar Mishra (2019), "Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone," https://diagrid.org/resources/1417.

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