Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

By Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2, Snehasis Jana2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

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Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes. First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to susceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the treated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration in the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA (53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus) on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and (53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed that the identified microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The nearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). The results suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state.


1. Metri BC, Jyothi P, Peerapur BV (2013) Antibiotic resistance in Citrobacter spp. isolated from urinary tract infection. Urol Ann 5: 312-313.

2. Arens S, Verbist L (1997) Differentiation and susceptibility of Citrobacter isolates from patients in a university hospital. Clin Microbiol Infect 3: 53-57.

3. Drelichman V, Band JD (1985) Bacteremias due to Citrobacter diversus and Citrobacter freundii. Incidence, risk factors, and clinical outcome. Arch Intern Med 145: 1808-1810.

4. MacCulloch D, Menzies R, Cornere BM (1977) Endocarditis due to Citrobacter diversus developing resistance to cephalothin. N Z Med J 85: 182-183.

5. Williams WW, Mariano J, Spurrier M, Donnell HD Jr, Breckenridge RL Jr, et al. (1984) Nosocomial meningitis due to Citrobacter diversus in neonates: New aspects of the epidemiology. J Infect Dis 150: 229-235.

6. Mateos Rodríguez F, Pérez Moro E, Atienza Morales MP, Beato Pérez JL (2000) [Community-acquired bacteremic pneumonia due to Citrobacter diversus]. An Med Interna 17: 165-166.

7. Curless RG (1980) Neonatal intracranial abscess: Two cases caused by Citrobacter and a literature review. Ann Neurol 8: 269-272.

8. Jones RN, Jenkins SG, Hoban DJ, Pfaller MA, Ramphal R (2000) In vitro efficacy of six cephalosporins tested against Enterobacteriaceae isolated at 38 North American medical centers participating in the SENTRY antimicrobial surveillance program, 1997 - 1998. Int J Antimicrorb Agents 15: 111-118.

9. Lavigne JP, Defez C, Bouziges N, Mahamat A, Sotto A (2007) Clinical and molecular epidemiology of multidrug-resistant Citrobacter spp. infections in a French university hospital. Eur J Clin Microbiol Infect Dis 26: 439-441.

10. Badger JL, Stins MF, Kim KS (1999) Citrobacter freundii invades and replicates in human brain microvascular endothelial cells. Infect Immun 67: 4208-4215.

11. Samonis G, Anaissie E, Elting L, Bodey GP (1991) Review of Citrobacter bacteremia in cancer patients over a sixteen-year period. Eur J Clin Microbiol Infect Dis 10: 479-485.

12. Shih CC, Chen YC, Chang SC, Luh KT, Hsieh WC (1996) Bacteremia due to Citrobacter species: significance of primary intra-abdominal infection. Clin Infect Dis 23: 543-549.

13. Becker RO, Selden G (1998) The body electric: Electromagnetism and the foundation of life. (1stedn), William Morrow and Company. New York City,

14. Barnes RB (1963) Thermography of the human body. Science 140: 870-877.

15. Born M (1971) The Born-Einstein Letters. (1stedn), Walker and Company, New York.

16. Planck M (1903) Treatise on thermodynamics. (3rdedn), English translated by Alexander OGG, Longmans, Green, London (UK).

17. Einstein A (1905) Does the inertia of a body depend upon its energy-content? Ann Phys 18: 639-641.

18. Burr HS (1957) Bibliography of Harold Saxton Burr. Yale J Biol Med 30: 163-167.

19. Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendorf SK, et al. (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.

20. Movaffaghi Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations? Complement Ther Clin Pract 15: 35-37.

21. Rivera-Ruiz M, Cajavilca C, Varon J (2008) Einthoven’s string galvanometer: The first electrocardiograph. Tex Heart Inst J 35: 174-178.

22. Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.

23. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.

24. Trivedi MK, Tallapragada RM (2009) Effect of super consciousness external energy on atomic, crystalline and powder characteristics of carbon allotrope powders. Mater Res Innov 13: 473-480.

25. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.

26. Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 83: 138-143.

27. Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.

28. Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.

29. Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.

30. Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.

31. Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.

32. Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.

33. Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5: 150-163.

34. Pepperell C, Kus JV, Gardam MA, Humar A, Burrows LL, et al. (2002) Low-virulence Citrobacter species encode resistance to multiple antimicrobials. Antimicrob Agents Chemother 46: 3555-3560.

35. Brenner DJ, O’hara CM, Grimont PA, Janda JM, Falsen E, et al. (1999) Biochemical identification of Citrobacter species defined by DNA hybridization and description of Citrobacter gillenii sp. nov. (Formerly Citrobacter genomospecies 10) and Citrobacter murliniae sp. nov. (Formerly Citrobacter genomospecies 11). J Clin Microbiol 37: 2619-2624.

36. Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, et al. (2000) 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 38: 3623-3630.

37. Vandamme P, Pot B, Gillis M, de Vos P, Kersters K, et al. (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60: 407-438.

38. Lindstrom E, Mild KH, Lundgren E (1998) Analysis of the T cell activation signaling pathway during ELF magnetic field exposure, p56lck and [Ca2+] i-measurements. Bioeletrochem Bioenerg 46: 129-137.

Cite this work

Researchers should cite this work as follows:

  • Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/2472-128X.1000129

  • Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal; Snehasis Jana (2019), "Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen,"

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