Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobial sensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates. The minimum inhibitory concentration (MIC) study showed the alteration in MIC values of about 34.37% (11 out of 32) tested antimicrobials, after biofield treatment in clinical isolates of E. coli. Piperacillin/tazobactam was reported with improved sensitivity and four-fold decrease in the MIC value (64 to ≤16 µg/mL) in LS 42, as compared with the control. Amoxicillin/k-clavulanate reported with improved sensitivity pattern from resistance to susceptible, with two-fold decrease in MIC value (>16/8 to ≤8/4 µg/mL) in biofield treated LS 51. Further, biochemical study showed 24.24% alteration (8 out of 33) in tested biochemical reactions after treatment among four isolates of E. coli as compared to the control. A change in biotype number (7774 4272) was reported as compared to the control, (7311 4012), with new organism identified as Klebsiella pneumoniae in biofield treated LS 13 with respect to the control organism, E. coli. Overall, data suggested that Mr. Trivedi’s biofield energy treatment can be applied to alter the antimicrobial sensitivity, biochemical reactions and biotype number of E. coli.
 Belanger L, Garenaux A, Harel J, Boulianne M, Nadeau E, et al. (2011) Escherichia coli from animal reservoirs as a potential source of human extraintestinal pathogenic E. coli. FEMS Immunol Med Microbiol 62: 1-10.
 Sharon P (2002) E. coli: Environmental health issues of VTEC O157. London; New York: Spon Press.
 Kaper JB, Nataro JP, Mobley HL (2004) Pathogenic Escherichia coli. Nat Rev Microbiol 2: 123-140.
 Furyk JS, Swann O, Molyneux E (2011) Systematic review: Neonatal meningitis in the developing world. Trop Med Int Health 16: 672-679.
 Sahm DF, Thornsberry C, Mayfield DC, Jones ME, Karlowsky JA (2001) Multidrug-resistant urinary tract isolates of Escherichia coli: Prevalence and patient demographics in the United States in 2000. Antimicrob Agents Chemother 45: 1402-1406.
 Coates A, Hu Y, Bax R, Page C (2002) The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov 1: 895-910.
 Santillo VM, Lowe FC (2007) Cranberry juice for the prevention and treatment of urinary tract infections. Drugs Today (Barc) 43: 47-54.
 Lucchetti G, de Oliveira RF, Goncalves JP, Ueda SM, Mimica LM, et al. (2013) Effect of spiritist "passe" (spiritual healing) on growth of bacterial cultures. Complement Ther Med 21: 627-632.
 NIH, National Center for Complementary and Alternative Medicine. CAM Basics. Publication 347. [October 2, 2008]. Available at: http://nccam.nih.gov/health/whatiscam/.
 Jahn RG, Dunne BJ (1988) Margins of Reality: The role of consciousness in the physical World. San Diego, CA: Harcourt Brace Jovanovich.
 Rosch Paul J (2009) Bioelectromagnetic and subtle energy medicine. The Interface between mind and matter. Longevity, regeneration and optimal health, New York Academy of Science.
 Garland SN, Valentine D, Desai K, Li S, Langer C, et al. (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
 Peck SD (1998) The efficacy of therapeutic touch for improving functional ability in elders with degenerative arthritis. Nurs Sci Q 11: 123-132.
 Turner JG, Clark AJ, Gauthier DK, Williams M (1998) The effect of therapeutic touch on pain and anxiety in burn patients. J Adv Nurs 28: 10-20.
 Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 8: 138-143.
 Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
 Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on phenotypic and genotypic characteristic of Provindencia rettgeri. Mol Biol 4: 129.
 Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
 Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.
 Thenmozhi S, Moorthy K, Sureshkumar BT, Suresh M (2014) Antibiotic resistance mechanism of ESBL producing Enterobacteriaceae in clinical field: A review. Int J Pure Appl Biosci 2: 207-226.
 Paterson DL, Bonomo RA (2005) Extended-spectrum β-lactamases: A clinical update. Clin Microbiol Rev 18: 657-686.
 Wallen L, Zeller WP, Goessler M, Connor E, Yogev R (1983) Single-dose amikacin treatment of first childhood E. coli lower urinary tract infections. J Pediatr 103: 316-319.
 Davies J, Wright GD (1997) Bacterial resistance to aminoglycoside antibiotics. Trends Microbiol 5: 234-240.
 Lee J, Oh CE, Choi EH, Lee HJ (2013) The impact of the increased use of piperacillin/tazobactam on the selection of antibiotic resistance among invasive Escherichia coli and Klebsiella pneumoniae isolates. Int J Infect Dis 17: e638-e643.
 Pitout JD (2012) Extraintestinal pathogenic Escherichia coli: An update on antimicrobial resistance, laboratory diagnosis and treatment. Expert Rev Anti Infect Ther 10: 1165-1176.
 Retamar P, Lopez-Cerero L, Muniain MA, Pascual A, Rodriguez-Bano J, et al. (2013) Impact of the MIC of piperacillin-tazobactam on the outcome of patients with bacteremia due to extended-spectrum-beta-lactamase-producing Escherichia coli. Antimicrob Agents Chemother 57: 3402-3404.
 Farasat T, Bilal Z, Yunus F (2012) Isolation and biochemical identification of Escherichia coli from wastewater effluents of food and beverage industry. J Cell Mol Biol 10: 13-18.
 Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL (2015) Trends in the use of complementary health approaches among adults: Unites States, 2002-2012. National health statistics reports; no 79. Hyattsville, MD: National Center for Health Statistics.
 Rubik B, Brooks AJ, Schwartz GE (2006) In vitro effect of Reiki treatment on bacterial cultures: Role of experimental context and practitioner wellbeing. J Altern Complement Med 12: 7-13.
 Chein CH, Tsuei JJ, Lee SC, Huang YC, Wei YH (1991) Effect of emitted bioenergy on biochemical function of cells. Am J Chin Med 19: 285-292.
 Movaffaghi Z, Hassanpoor M, Farsi M, Hooshmand P, Abrishami F (2006) Effects of therapeutic touch on blood hemoglobin and hematocrit level. J Holist Nurs 24: 41-48.
 Olson M, Sneed N, Lavia M, Virella G, Bonadonna R, et al. (1997) Stress-induced immunosuppression and therapeutic touch. Altern Ther Health Med 3: 68-74.
 Wirth DP (1990) The effect of non-contact therapeutic touch on the healing rate of full thickness dermal wounds. Subtle Energies 1: 1-20.
Cite this work
Researchers should cite this work as follows:
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana. Use of Energy Healing Medicine Against Escherichia coli for Antimicrobial Susceptibility, Biochemical Reaction and Biotyping. American Journal of Bioscience and Bioengineering. Vol. 3, No. 5, 2015, pp. 99-105. doi: 10.11648/j.bio.20150305.23