Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri

By Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2, Snehasis Jana2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

Published on

Abstract

Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.

References

[1] Holt JG (1994) Bergey's manual of determinative bacteriology. (9thedn), Williams & Wilkins, Baltimore, MD.

[2] Armbruster CE, Smith SN, Yep A, Mobley HL (2014) Increased incidence of urolithiasis and bacteremia during Proteus mirabilis and Providencia stuartii coinfection due to synergistic induction of urease activity. J Infect Dis 209: 1524-1532.

[3] Manos J, Belas R (2006) The genera Proteus, Providencia, and Morganella. Prokaryotes 6: 245-269.

[4] Obayes HS, GAbd F (2013) Pathogenesis of Providencia rettgeri in mice. Journal of Babylon University/Pure and Applied Sciences 21: 2785-2800.

[5] Yoh M, Matsuyama J, Ohnishi M, Takagi K, Miyagi H, Mori K, et al. (2005) Importance of Providencia species as a major cause of travellers' diarrhoea. J Med Microbiol 54: 1077-1082.

[6] OʼHara CM, Brenner FW, Miller JM (2000) Classification, identification, and clinical significance of Proteus, Providencia and Morganella. Clin Micro Rev 13: 534-546.

[7] Matsuura M, Nakazawa H, Inoue M, Mitsuhashi S (1980) Purification and biochemical properties of beta-lactamase produced by Proteus rettgeri. Antimicrob Agents Chemother 18: 687-690.

[8] Koithan M (2009) Introducing complementary and alternative therapies. J Nurse Pract 5: 18-20.

[9] Prakash S, Chowdhury AR, Gupta A (2015) Monitoring the human health by measuring the biofield "aura": An overview. IJAER 10: 27637-27641.

[10] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.

[11] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.

[12] Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.

[13] Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.

[14] Trivedi MK, Tallapragada RR (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28, 31.

[15] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.

[16] Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.

[17] Penner JL, Preston MA (1980) Differences among Providencia species in their in vitro susceptibilities to five antibiotics. Antincrob Agents Chemother 18: 868-871.

[18] Stock I, Wiedemann B (1998) Natural antibiotic susceptibility of Providencia stuartii, P. rettgeri, P. alcalifaciens, and P. rustigianii strains. J Med Microbiol 47: 629-642.

[19] Mino Y, Kitano S, Morimoto S, Ogihara T (1997) Urinary bacteria in elderly patients with urinary incontinence and low levels of daily activity. Nihon Ronen Igakkai Zasshi 34: 1004-1008.

[20] Farmer JJ 3rd, Hickman FW, Brenner DJ, Schreiber M, Rickenbach DG, (1977) Unusual Enterobacteriaceae: "Proteus rettgeri" that "change" into Providencia stuartii. J Clin Microbiol 6: 373-378.

[21] Brenner DJ, Farmer III JJ, Fanning GR, Steigerwalt AG, Klykken P, et al. (1978) Deoxyribonucleic acid relatedness of Proteus and Providencia species. Int J Syst Bacteriol 28: 269-282.

[22] Brenner Don J, Krieg Noel R, Staley James R (2005) Manual® of Systematic Bacteriology: The Proteobacteria, Part B: The Gammaproteobacteria George Garrity, Springer Science & Business Media.

[23] Senior BW (1997) Media and tests identification of bacteria to simplify the recognition and members of the Proteeae. J Med Microbiol 46: 39-44.

[24] Lindstrom E, Mild KH, Lundgren E (1998) Analysis of the T cell activation signaling pathway during ELF magnetic field exposure, p56lck and [Ca2+]i-measurements. Bioeletrochem Bioenerg 46: 129-137.

Cite this work

Researchers should cite this work as follows:

  • Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana. Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri. American Journal of Health Research. Vol. 3, No. 6, 2015, pp. 344-351.doi: 10.11648/j.ajhr.20150306.15
     

  • Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal; Snehasis Jana (2019), "Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri," https://diagrid.org/resources/1540.

    BibTex | EndNote

Tags