Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone

By Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1, Gopal Nayak1, Khemraj Bairwa2, Snehasis Jana2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

Published on


Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Subsequently, both the samples were assessed using numerous analytical techniques. The X-ray diffractograms (XRD) showed the halo patterns of XRD peaks in both the samples. The particle size analysis exhibited about 4.70% and 17.58% increase in the d50 (average particle size) and d99 (particle size below which 99% particles are present), respectively of treated bio peptone as compared to the control. The surface area analysis revealed the 253.95% increase in the specific surface area of treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis showed the 29.59% increase in the melting temperature of treated bio peptone sample as compared to the control. Thermogravimetric analysis (TGA) showed the increase in onset of degradation temperature by 3.31% in the treated sample with respect to the control. The Fourier transform infrared (FT-IR) study revealed the changes in the wavenumber of functional groups such as O-H stretching from 3066 cm-1 to 3060 cm-1; C-H stretching from 2980, 2893, and 2817 cm-1 to 2970, 2881, and 2835 cm-1, respectively; N-H bending from 1589 cm-1 to 1596 cm-1; C=C stretching from 1533 cm-1 to 1525 cm-1; and P=O stretching from 1070 cm-1 to 1078 cm-1 in treated sample as compared to the control. The UV-vis spectroscopy showed the similar patterns of absorbance maxima (λmax) i.e. at 259 nm and 257 nm in both the control and treated samples, respectively. Overall, the analytical results suggested that Mr. Trivedi’s biofield energy treatment has substantial effect on physicochemical and spectral properties of bio peptone. Owing to this, the treated bio peptone might be more effective as culture medium than the corresponding control.


[1] Madigan M, Martinko J (2005) Brock biology of microorganisms. (11thedn), Prentice Hall. Upper saddle river, NJ, USA.

[2] Wade J, Graver M (2010) Chemically defined culture medium for neisseria. US 20100003740 A1.

[3] Schlegel HG, Zaborosch C, Kogut M (1993). General Microbiology. (7thedn), Cambridge university press, New York, USA.

[4] Todar K (2000) Culture Media for the Growth of Bacteria. University of Wisconins-Madison.

[5] Atlas RM (2010) Handbook of Microbiological Media. (4thedn), CRC press, Taylor and Francis group, New York, USA.


[7] Rivera-Posada J, Caballes CF, Pratchett MS (2013) Lethal doses of oxbile, peptones and thiosulfate-citrate-bile-sucrose agar (TCBS) for Acanthaster planci; Exploring alternative population control options. Mar Pollut Bull 75: 133-139.

[8] Basu S, Pal A, Desai PK (2005) Quality control of culture media in a microbiology laboratory. Ind J Med Microbiol 23: 159-163.

[9] Ellaiah P, Srinivasulu B, Adinarayana K (2002) A review on microbial alkaline proteases. J Sci Ind Res 61: 690-704.

[10] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.

[11] Rindfleisch JA (2010) Biofield therapies: Energy medicine and primary care. Prim Care 37: 165-179.

[12] NIH, National center for complementary and alternative medicine. CAM basics. Publication 347. [October 2, 2008]. Available at:

[13] Jahn RG, Dunne BJ (1988) Margins of reality: The role of consciousness in the physical world. San Diego, CA: Harcourt Brace Jovanovich.

[14] Rosch PJ (2009) Bioelectromagnetic and subtle energy medicine. The Interface between mind and matter. Longevity, regeneration and optimal health, New York Academy of Science.

[15] Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of Biofield treatment on ginseng and organic blueberry yield. Agrivita, J Agric Sci 35: 22-29.

[16] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.

[17] Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.

[18] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, et al. (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.

[19] Trivedi MK, Patil S, Shettigar H, Singh R, Jana S (2015) An impact of biofield treatment on spectroscopic characterization of pharmaceutical compounds. Mod Chem appl 3: 159.

[20] Pavia DL, Lampman GM, Kriz GS (2001) Introduction to spectroscopy. (3rdedn), Thomson Learning, Singapore.

[21] Chauhan A, Chauhan P (2014) Powder XRD technique and its applications in science and technology. J Anal Bioanal Tech 5: 212.


[23] Gad SC (2008) Pharmaceutical manufacturing handbook: Production and processes. John Wiley & Sons, Inc., Publication, New Jersey, USA.

[24] Trivedi MK, Patil S, Mishra RK, Jana S (2015) thermal and physical properties of biofield treated bile salt and proteose peptone. J Anal Bioanal Tech 6: 256.

[25] Groza JR, Shackelford JF (2007) Materials processing handbook. Taylor and Francis group, CRC Press.

[26] Suttiponparnit K, Jiang J, Sahu M, Suvachittanont S, Charinpanitkul T, et al (2011) Role of surface area, primary particle size, and crystal phase on titanium dioxide nanoparticle dispersion properties. Nanoscale Res Lett 6: 27.

[27] Paradkar AR, Bakliwal S (2008). Biopharmaceutics and pharmacokinetics. (3rdedn), Pragati Books Pvt. Ltd., Pune, India.

[28] Kumar S, Tsai CJ, Nussinov R (2000) Factors enhancing protein thermostability. Protein Eng 13: 179-191.

[29] Ratta V (1999) Crystallization, morphology, thermal stability and adhesive properties of novel high performance semicrystalline polyimides. VT.

[30] Qi WH, Wang MP (2004) Size and shape dependent melting temperature of metallic nanoparticles. Mater Chem Phys 88: 280-284.

[31] DeVito SC, Farris CA (1997) Premanufacture notification: Chemistry assistance for submitters. John Wiley & Sons, INC., New York, USA.

[32] Spear RJ, Maksacheff M (1986) The relationship between ignition temperature and thermal stability for selected primary explosives. Thermochim Acta 105: 287-293.

[33] Smith BC (1998) Infrared spectral interpretation: A systematic approach. CRC Press.

Cite this work

Researchers should cite this work as follows:

  • Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana. Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone. Advances in Bioscience and Bioengineering. Vol. 3, No. 6, 2015, pp. 59-66. doi: 10.11648/

  • Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Khemraj Bairwa; Snehasis Jana (2019), "Characterization of Physicochemical and Spectroscopic Properties of Biofield Energy Treated Bio Peptone,"

    BibTex | EndNote