The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Trivedi’s biofield treatment, coded as the treated sample and another part was coded as the control. The impact of biofield treatment was analysed through various analytical techniques and results were compared with the control sample. The particle size data revealed 4.61% increase in the average particle size (d50) along with 2.66% reduction in the surface area of the treated sample as compared to the control. The X-ray diffraction studies revealed the amorphous nature of the fish peptone sample; however no alteration was found in the diffractogram of the treated sample with respect to the control. The Fourier transform infrared studies showed the alterations in the frequency of peaks corresponding to N-H, C-H, C=O, C-N, and C-OH, functional groups in the treated sample as compared to the control. The differential scanning calorimetry data revealed the increase in transition enthalpy (∆H) from -71.14 J/g (control) to -105.32 J/g in the treated sample. The thermal gravimetric analysis data showed the increase in maximum thermal degradation temperature (Tmax) from 213.31°C (control) to 221.38°C along with a reduction in the percent weight loss of the treated sample during the thermal degradation event. These data revealed the increase in thermal stability of the treated fish peptone and suggested that the biofield energy treatment may be used to improve the thermal stability of the heat sensitive compounds.
 Ferraro V, Cruz IB, Jorge RF, Malcata FX, Pintado ME, et al. (2010) Valorisation of natural extracts from marine source focused on marine by-products: A review. Food Res Int 43: 2221-2233.
 Dekkers E, Raghavan S, Kristinsson HG, Marshall MR (2011) Oxidative stability of mahi mahi red muscle dipped in tilapia protein hydrolysates. Food Chem 124: 640-645.
 Galvez RP, Berge JP (2013) By-products from fish processing: Focus on French industry. Utilization of fish waste. CRC Press, Taylor & Francis Group, New York.
 Kristinsson HG, Rasco BA (2000) Fish protein hydrolysates: Production, biochemical, and functional properties. Crit Rev Food Sci Nutr 40: 43-81.
 Clemente A (2000) Enzymatic protein hydrolysates in human nutrition. Trends Food Sci Technol 11: 254-262.
 Duarte J, Vinderola G, Ritz B, Perdigon G, Matar C (2006) Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation. Immunobiology 211: 341-350.
 Bridson EY, Brecker A (1970) Design and formulation of microbial culture media. Methods in microbiology. Academic Press, New York.
 Aspmo SI, Horn SJ, Eijsink VGH (2005) Hydrolysates from Atlantic cod (Gadus morhua L.) viscera as components of microbial growth media. Process Biochem 40: 3714-3722.
 Safari R, Motamedzadegan A, Ovissipour M, Regenstein JM, Gildberg A, et al. (2012) Use of hydrolysates from yellowfin tuna (Thunnus albacares) heads as a complex nitrogen source for lactic acid bacteria. Food Bioproc Tech 5: 73-79.
 Fallah M, Bahram S, Javadian SR (2015) Fish peptone development using enzymatic hydrolysis of silver carp by-products as a nitrogen source in Staphylococcus aureus media. Food Sci Nutr 3: 153-157.
 Ryan M, McEvoy E, Duignan S, Crowley C, Fenelon M, et al. (2008) Thermal stability of soy protein isolate and hydrolysate ingredients. Food Chem 108: 503-510.
 Dissanayake M, Vasiljevic T (2009) Functional properties of whey proteins affected by heat treatment and hydrodynamic high-pressure shearing. J Dairy Sci 92: 1387-1397.
 Nicorescu I, Riaublanc A, Loisel C, Vial C, Djelveh G, et al. (2009) Impact of protein self-assemblages on foam properties. Food Res Int 42: 1434-1445.
 Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, et al. (2015) Biofield treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.
 Saad M, Medeiros RD (2012) Distant healing by the supposed vital energy- scientific bases. Complementary therapies for the contemporary healthcare. InTech.
 Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
 Garland SN, Valentine D, Desai K, Li S, Langer C, et al. (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
 Peck SD (1998) The efficacy of therapeutic touch for improving functional ability in elders with degenerative arthritis. Nurs Sci Q 11: 123-132.
 Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
 Trivedi MK, Patil S, Harish S, Gangwar M, Jana S (2015) Biofield treatment: An alternative approach to combat multidrug-resistant susceptibility pattern of Raoultella ornithinolytica. Altern Integr Med 4: 193.
 Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and physical properties of biofield treated bile salt and proteose peptone. J Anal Bioanal Tech 6: 256.
 Amin S, Barnett GV, Pathak JA, Roberts CJ, Sarangapani PS (2014) Protein aggregation, particle formation, characterization and rheology. Curr Opin Colloid Interface Sci 19: 438-449.
 Khutoryanskiy VV, Nurkeeva ZS, Mun GA, Dubolazov AV (2004) Effect of temperature on aggregation/dissociation behavior of interpolymer complexes stabilized by hydrogen bonds. J Appl Polym Sci 93: 1946-1950.
 Aulton ME, Taylor KMG (2013) Aulton’s pharmaceutics: The design and manufacture of medicines. (4thedn), Churchill Livingstone, Elsevier.
 Suryanarayana C, Norton MG (2013) X-ray diffraction: A practical approach. Springer sciences and business media.
 Kose A, Oncel SS (2015) Properties of microalgal enzymatic protein hydrolysates: Biochemical composition, protein distribution and FTIR characteristics. Biotechnol Rep 6: 137-143.
 Lambert JB (1987) Introduction to organic spectroscopy. Macmillan, New York, USA.
 Snavely DL, Blackburn FR, Ranasinghe Y, Walters VA, del Riego MG (1992) Vibrational overtone spectroscopy of pyrrole and pyrrolidine. J Phys Chem 96: 3599-3605.
 Abood NA, Al-Hlfi JAH (2013) Theoretical study of structure and vibrational frequencies of pyrazole and its derivatives, and X-ray structure determination. Misan J Academic Studies 12: 29-40.
 Gill P, Moghadam TT, Ranjbar B (2010) Differential scanning calorimetry techniques: Applications in biology and nanoscience. J Biomol Tech 21: 167-193.
 He X, Cao W, Zhao Z, Zhang C (2013) Analysis of protein composition and antioxidant activity of hydrolysates from Paphia undulate. J Food Nutr Res 1: 30-36.
 Stodghill SP (2010) Thermal analysis- A review of techniques and applications in the pharmaceutical sciences. American Pharmaceutical Review 13.
 Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, et al (2015) Evaluation of the impact of biofield treatment on physical and thermal properties of casein enzyme hydrolysate and casein yeast peptone. Clin Pharmacol Biopharm 4: 138.
Cite this work
Researchers should cite this work as follows:
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana. Physical, Spectroscopic and Thermal Characterization of Biofield Treated Fish Peptone. European Journal of Biophysics. Vol. 3, No. 6, 2015, pp. 51-58. doi: 10.11648/j.ejb.20150306.12