Global emergence of Acinetobacter baumannii (A. baumannii) displays a mechanism of resistance to all existing antimicrobials. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern, minimum inhibitory concentration (MIC), biochemical reactions and biotype number of A. baumannii. A. baumannii cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 19606) number and stored according to the recommended storage protocols until needed for experiments. Two sets of ATCC samples were taken in this experiment and denoted as A and B. ATCC-A sample was revived and divided into two parts i.e. Gr.I (control) and Gr.II (revived) analyzed on day 5 and 10, respectively; likewise, ATCC-B was labeled as Gr.III (lyophilized) and was assessed on day 10. Gr.II and III were treated with Mr. Trivedi’s biofield and were analyzed for its antimicrobial sensitivity, MIC value, biochemical reactions and biotype number with respect to control. Experimental results showed the impact of biofield treatment directly onto the revived and lyophilized form of A. baumannii and found alteration both in qualitative and quantitative aspect as compared with untreated groups. These results showed altered sensitivity pattern of antimicrobials in biofield treated group as compared to control. Apart from altered MIC values, changes were also observed in biotype number of revived treated group as compared to control. These findings suggest that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to A. baumannii.
1. Baumann P, Doudoroff M, Stanier RY (1968) Study of the Moraxella group. I. Genus Moraxella and the Neisseria catarrhalis group. J Bacteriol 95: 58-73.
2. Peleg AY, Seifert H, Paterson DL (2008) Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21: 538-582.
3. Vila J, Pachón J (2012) Therapeutic options for Acinetobacter baumannii infections: an update. Expert Opin Pharmacother 13: 2319-2336.
4. Abbott GM, Cerqueira S, Bhuiyan AY (2013) Carbapenem resistance in Acinetobacter baumannii: laboratory challenges, mechanistic insights and therapeutic strategies. Expert Rev Anti Infect Ther 11: 395-409.
5. La Scola B1, Raoult D (2004) Acinetobacter baumannii in human body louse. Emerg Infect Dis 10: 1671-1673.
6. Chastre J, Trouillet JL (2000) Problem pathogens (Pseudomonas aeruginosa and Acinetobacter). Semin Respir Infect 15: 287-298.
7. Benor DJ (2002) Energy medicine for the internist. Med Clin North Am 86: 105-125.
8. Jonas WB, Crawford CC (2003) Science and spiritual healing: a critical review of spiritual healing, "energy" medicine, and intentionality. Altern Ther Health Med 9: 56-61.
9. Einstein A (1905) Does the inertia of a body depend upon its energy-content. Ann Phys 18: 639-641.
10. Trivedi MK, Patil S (2008) Impact of an External energy on Staphylococcus epidermis [ATCC-13518] in relation to antimicrobials susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 4: 230-235.
11. Trivedi MK, Patil S (2008) Impact of an external energy on Yersinia enterocolitica [ATCC-23715] in relation to antimicrobials susceptibility and biochemical reactions: an experimental study. Internet J Alternat Med 6: 13.
12. Trivedi MK, Bhardwaj Y, Patil S, Shettigar H, Bulbule A (2009) Impact of an external energy on Enterococcus faecalis [ATCC-51299] in relation to antimicrobials susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 5: 119-130.
13. Trivedi MK, Tallapragada RR (2008) A transcendental to changing metal powder characteristics. Metal Powder Rep 63: 22-28, 31.
14. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mat Sci 32: 471-479.
15. Trivedi MK, Tallapragada RR (2009) Effect of superconsciousness external energy on atomic, crystalline and powder characteristics of carbon allotrope powders. Mat Res Innov 13: 473-480.
16. Trivedi MK, Patil S, Tallapragada RM (2012) Thought Intervention through biofield changing metal powder characteristics experiments on powder characterisation at a PM Plant, Springer Berlin Heidelberg, Editor: Wei Deng, Lecture Notes in Electrical Engineering, Future Control and Automation 173: 247-252.
17. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of Biofield treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S11: 001.
18. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of Silicon, Tin and Lead powders. J Material Sci Eng 2: 125.
19. Trivedi MK, Patil S, Tallapragada RM (2014) Atomic, crystalline and powder characteristics of treated zirconia and silica powders. J Material Sci Eng 3: 144.
20. Trivedi MK, Patil S, Tallapragada RMR (2015) Effect of biofield treatment on the Physical and Thermal Characteristics of Aluminium Powders. Ind Eng Manage 4:151.
21. Shinde V, Sances F, Patil S, Spence A (2012) Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basic and Appl Sci 6: 100-105.
22. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield Treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
23. Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 8: 138-143.
24. Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11:154-162.
25. Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
26. Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScan WalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J Clin Microbiol 51: 1548-1554.
27. Gomaa FM, Tawakol WM, Abo El-Azm FI (2014) Phenotypic and genotypic detection of some antimicrobial resistance mechanisms among multidrug-resistant Acinetobacter baumannii isolated from immunocompromised patients in Egypt. Egypt J Med Microbiol 23: 99-111.
28. Paterson DL, Bonomo RA (2005) Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev 18: 657-686.
29. Bratu S, Landman D, Martin DA, Georgescu C, Quale J (2008) Correlation of antimicrobial resistance with beta-lactamases, the OmpA-like porin, and efflux pumps in clinical isolates of Acinetobacter baumannii endemic to New York City. Antimicrob Agents Chemother 52: 2999-3005.
30. Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, et al. (2007) Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 51: 3471-3484.
31. Walther-Rasmussen J, Høiby N (2006) OXA-type carbapenemases. J Antimicrob Chemother 57: 373-383.
32. Higgins PG, Wisplinghoff H, Stefanik D, Seifert H (2004) In vitro activities of the ß-lactamase inhibitors clavulanic acid, sulbactam, and tazobactam alone or in combination with ß-lactams against epidemiologically characterized multidrug-resistant Acinetobacter baumannii strains. Antimicrob Agents Chemother 48: 1586-1592.
Cite this work
Researchers should cite this work as follows:
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Effect of Biofield Treatment on Antimicrobials Susceptibility Pattern of Acinetobacter baumannii – An Experimental Study. J Clin Diagn Res 3:117. doi:10.4172/2376-0311.1000117