The stable isotope ratio analysis is widely used in several scientific fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. Resorcinol is one of the most versatile chemicals used for the synthesis of several pharmaceuticals, dyes, polymers, organic compounds, etc. The current research work was designed to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of 13C/12C or 2H/1H or 17O/16O (PM+1/PM) and 18O/16O (PM+2/PM) in resorcinol using Gas chromatograph - mass spectrometry (GC-MS) technique. Resorcinol was divided into two parts - one part was control and another part was considered as biofield energy treated sample. The biofield energy treatment was accomplished through unique biofield energy transmission by Mr. Mahendra Kumar Trivedi (also called as The Trivedi Effect®). T1, T2, T3, and T4 were denoted by different time interval analysis of the biofield treated resorcinol in order to understand the influence of the biofield energy treatment on isotopic abundance ratio with respect to the time. The GC-MS spectra of the both control and biofield treated resorcinol exhibited the presence of molecular ion peak [M+] at m/z 110 (calculated 110.04 for C6H6O2) along with major fragmented peaks at m/z 82, 81, 69, 53, and 39. The relative peak intensities of the fragmented ions in biofield treated resorcinol (particularly T2) was significantly changed with respect to the control sample. The stable isotope ratio analysis in resorcinol using GC-MS revealed that the percentage change of the isotopic abundance ratio of PM+1/PM was increased in the biofield treated resorcinol at T1, T2, T3 and T4 by 1.77%, 165.73%, 0.74%, and 6.79%, respectively with respect to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield treated resorcinol at T2, T3, and T4 were enhanced by 170.77%, 3.08%, and 12.31%, respectively with respect to the control sample. Briefly, 13C, 2H, 17O contributions from (C6H6O2)+ to m/z 111 and 18O contribution from (C6H6O2)+ to m/z 112 for the biofield treated resorcinol at T2 and T4 were significantly altered as compared to the control sample. For this reasons, biofield treated resorcinol might exhibit altered physicochemical properties like diffusion velocity, mobility and evaporation rate, reaction rate, binding energy, and stability. Biofield treated resorcinol could be valuable in pharmaceutical and chemical industries as intermediates during the preparation of pharmaceuticals and chemical compounds by altering its physicochemical properties, the reaction rate and selectivity, the study of the reaction mechanism and facilitating in designing extremely effective and specific enzyme inhibitors.
1. Gannes LZ, del Rio C, Koch P (1998) Natural abundance variations in stable isotopes and their potential uses in animal physiological ecology. Comp Biochem Physiol A Mol Integr Physiol 119: 725-737.
2. Muccio Z, Jackson GP (2009) Isotope Ratio Mass Spectrometry. Analyst 134: 213-222.
3. Ben-David M, Flaherty EA (2012) Stable isotopes in mammalian research: a beginner’s guide. Journal of Mammalogy 93: 312-328.
4. Schellekens RC, Stellaard F, Woerdenbag HJ, Frijlink HW, Kosterink JG (2011) Applications of stable isotopes in clinical pharmacology. Br J Clin Pharmacol 72: 879-897.
5. Han B, Kang HM, Eskin E (2009) Rapid and accurate multiple testing correction and power estimation for millions of correlated markers. PLoS Genet 5: e1000456.
7. Vanhaecke F, Kyser K (2012) Isotopic composition of the elements In Isotopic Analysis: Fundamentals and applications using ICP-MS( 1stedn) Wiley-VCH GmbH & Co. KGaA, Weinheim.
8. Plekhanov VG (2004) Applications of the isotopic effect in solids (1stedn) Springer-Verlag Berlin Heidelberg, New York.
9. Asperger S (2003) Chemical Kinetics and Inorganic Reaction Mechanisms, Springer science, Business media, New York.
10. Meier-Augenstein W (1999) Applied gas chromatography coupled to isotope ratio mass spectrometry. J Chromatogr A 842: 351-371.
11. Smith RM (2004) Understanding Mass Spectra: A Basic Approach (2ndedn) John Wiley & Sons, Inc.
12. Jürgen H. Gross (2004) Mass Spectrometry (2ndedn) Springer, Berlin.
13. Raymond EM, John FJT (2010) Practical Aspects of Trapped Ion Mass Spectrometry, Volume IV: Theory and instrumentation, CRC press, Taylor and Francis Group, Boca Raton.
14. Mass Spectrometry: Isotope Effects, ChemWiki: The Dynamic Chemistry Hypertext.
15. Durairaj RB (2005) Resorcinol: Chemistry, technology and applications. Springer-Verlag, Berlin, Germany.
16. Dressler H (1994) Resorcinol Its Uses and Derivatives, Springer science, New York.
17. Hahn S, Kielhorn J, Koppenhöfer J, Wibbertmann A, Mangelsdorf I (2006) Resorcinol. Concise international chemical assessment document 71. WHO Press, World Health Organization, Geneva, Switzerland.
18. Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, et al. (2015) Characterisation of physical, spectral and thermal properties of biofield treated resorcinol. Organic Chem Curr Res 4: 146.
19. Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic characterization of disulfiram and nicotinic acid after biofield treatment. J Anal Bioanal Tech 6: 265.
20. Trivedi MK, Patil S, Shettigar H, Singh R, Jana S (2015) An impact of biofield treatment on spectroscopic characterization of pharmaceutical compounds. Mod Chem Appl 3: 159.
21. Trivedi MK , Tallapragada RM , Branton A , Trivedi D, Nayak G, et al. (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
22. Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Physical, thermal, and spectroscopic characterization of biofield energy treated methyl-2-naphthyl ether. J Environ Anal Chem 2: 162.
23. Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Physicochemical and spectroscopic characterization of biofield treated triphenyl phosphate. American Journal of Applied Chemistry 3: 168-173.
24. Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Physicochemical and spectroscopic characteristics of biofield treated p-chlorobenzophenone. American Journal of Physical Chemistry 4: 48-57.
25. Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Potential impact of biofield energy treatment on the atomic, physical and thermal properties indium powder. J Material Sci Eng 4: 198.
26. Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, et al. (2015) The potential impact of biofield treatment on physical, structural and mechanical properties of stainless steel powder. J Appl Mech Eng 4:173.
27. Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, et al. (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
28. Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, et al. (2015) Effect of biofield energy treatment on chlorophyll content, pathological study, and molecular analysis of cashew plant (Anacardium occidentale L.). Journal of Plant Sciences 3: 372-382.
29. Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
30. Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro Evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
31. Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Antimicrobial susceptibility pattern, biochemical characteristics and biotyping of Salmonella paratyphi A: An impact of biofield treatment. Clin Microbiol 4: 215.
32. Trivedi MK, Branton A, Trivedi D, Shettigar H, Gangwar M, et al. (2015) antibiogram typing and biochemical characterization of Klebsiella pneumonia after biofield treatment. J Trop Dis 3: 173.
33. Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Effect of biofield treated energized water on the growth and health status in chicken (Gallus gallus domesticus). Poult Fish Wildl Sci 3: 140.
34. Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Evaluation of phenotyping and genotyping characteristic of Shigella sonnei after biofield treatment. J Biotechnol Biomater 5: 196.
35. Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, et al. (2015) Investigation of isotopic abundance ratio of biofield treated phenol derivatives using gas chromatography-mass spectrometry. J Chromatograph Separat Techniq S6: 003.
36. Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, et al. (2015) Influence of biofield energy treatment on isotopic abundance ratio in aniline derivatives. Mod Chem appl 3: 168.
37. Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, et al. (2015) Isotopic abundance analysis of biofield treated benzene, toluene and p-xylene using Gas Chromatography-Mass Spectrometry (GC-MS). Mass Spectrom Open Access 1: 102.
38. Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, et al. (2015) Evaluation of isotopic abundance ratio of naphthalene derivatives after biofield energy treatment using Gas Chromatography-Mass Spectrometry. American Journal of Applied Chemistry 3: 194-200.
39. Meija J, Coplen TB, Berglund M, Brand WA, De Bievre P, et al. (2016) Isotopic compositions of the elements 2013 (IUPAC technical Report). Pure Appl Chem 88: 293-306.
40. Sparkman OD (2010) MS Solutions #5: The Role of Isotope Peak Intensities Obtained Using Mass Spectrometry in Determining an Elemental Composition, Part 1. Separation Science.
41. (2011) Resorcinol, National Institute of Standards and Technology, Material Measurement Laboratory.
42. Eileen Day McKusick (2015) How Plasma, Ether, Electric Universe Theory & Schumann Resonance Relate to Healing. Crowrising.
44. Rogers M (1989) "Nursing: A Science of Unitary Human Beings." In J.P. Riehl-Sisca (eds.) Conceptual Models for Nursing Practice (3rdedn) Norwark: Appleton & Lange.
45. Rubik B (2002) The biofield hypothesis: its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
46. Warber SL, Cornelio D, Straughn J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
48. Domogatskii GV, Nadezhin DK (1978) Neutrino production of bypassed isotopes, and the possible role of neutrinos in nucleosynthesis. Astron Zh 55: 516-530.
49. Jenkins L (2011) Healing in the present moment. Catholic News Agency.
50. Jean de Climont (2016) The worldwide list of dissident scientists. Science.
51. Klupenger MR (2015) An investigation into the effect of human intention on the weather, Energy Medicine University, Sausalito, California, USA.
53. Carr Jr. RW, Walters WD (1966) The hydrogen isotope effect in the thermal decomposition of cyclobutane, J Am Chem Soc 88: 884-887.
54. Lomas JS, Thorne MP (1982) Structure and isotope effects upon the thermal decomposition of carbamates of highly congested tertiary alcohols. J Chem Soc, Perkin Trans 2: 221-226.
55. Makhatadze GI, Clore GM, Gronenborn AM (1995) Solvent isotope effect and protein stability. Nat Struct Biol 2: 852-855.
57. Schramm VL (1998) Enzymatic transition states and transition state analog design. Annu Rev Biochem 67: 693-720.
58. Cleland WW (2003) The use of isotope effects to determine enzyme mechanisms. J Biol Chem 278: 51975-51984.
Cite this work
Researchers should cite this work as follows:
Mahendra Kumar T, Alice B, Dahryn T, Gopal N, Parthasarathi P, Snehasis J (2016) Evaluation of the Isotopic Abundance Ratio in Biofield Energy Treated Resorcinol Using Gas Chromatography-Mass Spectrometry Technique. Pharm Anal Acta 7: 481. doi: 10.4172/2153-2435.1000481
Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Parthasarathi Panda; Snehasis Jana (2019), "Evaluation of the Isotopic Abundance Ratio in Biofield Energy Treated Resorcinol Using Gas Chromatography-Mass Spectrometry Technique," https://diagrid.org/resources/1837.