The objective of the current study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of PM+1/PM, PM+2/PM, PM+3/PM and PM+4/PM in p-DCB using gas chromatography-mass spectrometry (GC-MS). The p-DCB was divided into two parts - one part was control sample, and another part was considered as the treated sample which was subjected to biofield energy treatment (The Trivedi Effect®). T1, T2, T3, and T4 were referred the biofield treated p-DCB having analyzed at different time intervals. The GC-MS analysis of both the control and biofield treated p-DCB indicated the presence of the parent molecular ion peak at m/z 146 along with four major fragmentation peaks at m/z 111, 75, 55 and 50. The relative peak intensities of the fragmented ions in the biofield treated p-DCB were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of PM+1/PM at T1, T2, T3, and T4 (biofield energy treated p-DCB) was significantly increased by 10.87, 83.90, 225.16, and 241.15%, respectively as compared to the control sample. Consequently, the percentage change in the isotopic abundance ratio of PM+2/PM at T1, T2, and T3 (biofield energy treated p-DCB) was enhanced by 4.55, 9.49, and 1.80%, respectively as compared to the control sample. Beside these, another two isotopic molecular ion peaks at m/z 149 and 150 were found in the GS-MS spectra due to arise from the contributions of various combinations of 2H, 13C, and 37Cl. The isotopic abundance ratios of PM+3/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 15.14, 82.57, 192.43, and 218.31%, respectively as compared to the control sample. Similarly, the PM+4/PM in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 13.80, 86.66, 186.13, and 204.29%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C), PM+2/PM (37Cl/35Cl), for PM+3/PM and PM+4/PM (the probable combinations of 2H/1H, 13C/12C, and 37Cl/35Cl) were significantly enhanced in the biofield energy treated p-DCB. The biofield treated p-DCB has shown improved isotopic abundance ratios that might have altered the physicochemical properties, thermal properties and rate of reaction. Biofield treated p-DCB might be useful in pharmaceutical and chemical industries as intermediates during the manufacturing of pharmaceuticals and chemicals by monitoring the rate of chemical reaction.
 Rossberg M, Lendle W, Pfleiderer G, Togel A, Dreher EL, Langer E, Rassaerts H, Kleinschmidt P, Strack H, Cook R, Beck U, Lipper KA, Torkelson TR, Löser E, Beutel KK, Mann T (2006) Chlorinated hydrocarbons. Ullmann's Encyclopedia of Industrial Chemistry. (7th Edn), Wiley-VCH, Verlag, Germany.
 National Pesticide Information Center - Parachlorobenzene general fact sheet. Retrieved in 02 April 2016.
 Fahey DR, Ash CE (1991) Mechanism of poly (p-phenylene sulfide) growth from p-dichlorobenzene and sodium sulphide. Macromolecules 24: 4242-4249.
 Agency for Toxic Substances and Disease Registry (ATSDR) (1998) Toxicological Profile for 1, 4-dichlorobenzene (Update). Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA.
 U.S. Department of Health and Human Services (1993) Hazardous Substances Data Bank (HSDB, online database). National Toxicology Information Program, National Library of Medicine, Bethesda.
 Griebler C, Adrian L, Meckenstock RU, Richnow HH (2004) Stable carbon isotope fractionation during aerobic and anaerobic transformation of trichlorobenzene. FEMS Microbiol Ecol 48: 313-321.
 Mackinson FW, Stricoff RS, Partridge LJ, Little AD (1981) Occupational Health Guidelines for Chemical Hazards. DHHS (NIOSH) Publication No. 81-123, Washington, DC, U.S.
 Djohan D, Yu J, Connell D, Christensen E (2007) Health risk assessment of chlorobenzenes in the air of residential houses using probabilistic techniques. J Toxicol Environ Health A 70: 1594-1603.
 Ogata M, Taguchi T, Hirota N, Shimada Y, Nakae S (1991) Quantitation of urinary chlorobenzene metabolites by HPLC: Concentrations of 4-chlorocatechol and chlorophenols in urine and of chlorobenzene in biological specimens of subjects exposed to chlorobenzene. Int Arch Occup Environ Health 63: 121-128.
 Winderl C, Penning H, von Netzer F, Meckenstock RU, Lueders T (2010) DNA-SIP identifies sulfate-reducing Clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment. The ISME Journal 4: 1314-1325.
 Muccio Z, Jackson GP (2009) Isotope ratio mass spectrometry. Analyst 134: 213-222.
 Ben-David M, Flaherty EA (2012) Stable isotopes in mammalian research: a beginner's guide. Journal of Mammalogy 93: 312-328.
 Scott, KM, Fox, G, Girguis PR (2011) Measuring isotope fractionation by autotrophic microorganisms and enzymes. Methods Enzymol 494: 281-299.
 Morgan JLL, Skulan JL, Gordon GW, Romaniello SJ, Smith SM, Anbar AD (2012) Rapidly assessing changes in bone mineral balance using natural stable calcium isotopes. Proc Natl Acad Sci USA 109: 9989-9994.
 Gevaert K, Impens F, Ghesquière B, Van Damme P, Lambrechts A, Vandekerckhove J (2008) Stable isotopic labeling in proteomics. Proteomics 8: 4873-85.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Evaluation of isotopic abundance ratio of naphthalene derivatives after biofield energy treatment using gas chromatography-mass spectrometry. American Journal of Applied Chemistry 3: 194-200.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Isotopic abundance analysis of biofield treated benzene, toluene and p-xylene using gas chromatography-mass spectrometry (GC-MS). Mass Spectrom Open Access 1: 102.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Influence of biofield energy treatment on isotopic abundance ratio in aniline derivatives. Mod Chem appl 3: 168.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Quantitative determination of isotopic abundance ratio of 13C, 2H, and 18O in biofield energy treated ortho and meta toluic acid isomers. American Journal of Applied Chemistry 3: 217-223.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Determination of isotopic abundance of 2H, 13C, 18O, and 37Cl in biofield energy treated dichlorophenol isomers. Science Journal of Analytical Chemistry 4: 1-6.
 Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendor SK, Oschman JL, Yount GL (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
 Warber SL, Cornelio D, Straughn J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
 Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
 Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Physical, thermal and spectroscopic studies on biofield treated p-dichlorobenzene. Biochem Anal Biochem 4: 204.
 Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
 Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mondal S, Jana S (2015) Impact of biofield energy treatment on soil fertility. Earth Sciences 4: 275-279.
 Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mondal S, Jana S (2015) Evaluation of biochemical marker - Glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience. 3: 243-248.
 Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mondal S, Jana S (2015) Antibiogram, biochemical reactions and genotyping characterization of biofield treated Staphylococcus aureus. American Journal of BioScience. 3: 212-220.
 Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015) Physical, thermal, and spectroscopic characterization of biofield energy treated potato micropropagation medium. American Journal of Bioscience and Bioengineering 3: 106-113.
 Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6: 395.
 Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
 Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
 Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
 Smith RM (2004) Understanding Mass Spectra: A Basic Approach, Second Edition, John Wiley & Sons, Inc, ISBN 0-471-42949-X.
 Taylor PDP (1998) Isotopic compositions of the elements 1997. J Phys Chem Ref Data 27: 1275.
 Weisel CP, Park S, Pyo H, Mohan K, Witz G (2003) Use of stable isotopically labeled benzene to evaluate environmental exposures. J Expo Anal Environ Epidemiol 13: 393-402.
 Jürgen H. Gross Mass Spectrometry: A Textbook; Springer: Berlin, 2nd Edn. 2004.
 Tsimeli K, Triantis TM, Dimotikali D, Hiskia A (2008) Development of a rapid and sensitive method for the simultaneous determination of 1, 2-dibromoethane, 1, 4-dichlorobenzene and naphthalene residues in honey using HS-SPME coupled with GC-MS. Anal Chim Acta 617: 64-71.
 Gordon J (1998) Inside informatics, cambridgesoft. com Article ID: Isotopic Abundance.
 Johnstone RAW, Rose ME (1996) Mass Spectrometry for Chemists and Biochemists, 2nd Edition. Cambridge university press.
 Wieser ME (2006) Atomic weights of the elements 2005. Pure Appl Chem 78: 2051-2066.
 Vanhaecke F, Kyser K (2012) Isotopic composition of the elements In Isotopic Analysis: Fundamentals and applications using ICP-MS (1 stedn), Edited by Vanhaecke F, Degryse P. Wiley-VCH GmbH & Co. KGaA, Weinheim.
 Asperger S (2003) Chemical Kinetics and Inorganic Reaction Mechanisms Springer science + Business media, New York.
 Lomas JS, Thorne MP (1982) Structure and isotope effects upon the thermal decomposition of carbamates of highly congested tertiary alcohols. J Chem Soc, Perkin Trans 2 221-226.
 www.nobelprize.org/nobel_prizes/physics/laureates/2015/advanced-physicsprize2015. pdf.
 Balantekin AB (2013) Neutrinos and rare isotopes Journal of Physics: Conference Series 445 012022.
Cite this work
Researchers should cite this work as follows:
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Kalyan Kumar Sethi, Snehasis Jana. Determination of Isotopic Abundance Ratio of Biofield Energy Treated 1,4-Dichlorobenzene Using Gas Chromatography-Mass Spectrometry (GC-MS). Modern Chemistry. Vol. 4, No. 3, 2016, pp. 30-37. doi: 10.11648/j.mc.20160403.11
Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Kalyan Kumar Sethi; Snehasis Jana (2019), "Determination of Isotopic Abundance Ratio of Biofield Energy Treated 1,4-Dichlorobenzene Using Gas Chromatography-Mass Spectrometry (GC-MS)," https://diagrid.org/resources/1849.