In vitro Assessment of the Biofield Treated Test Item on Cardiac Function Using Rat Cardiomyocytes Cell Line (H9c2) via Multiparametric Analysis

By Mahendra Kumar Trivedi1, Snehasis Jana2

1. Trivedi Global Inc. 2. Trivedi Science Research Laboratory Pvt. Ltd.

Published on

Abstract

Introduction: Heart disorders are the major concern of population health worldwide. According to WHO estimates 2018, 17.9 million peoples were died due to cardiovascular disorders.

Aim: The aim of this study was to investigate the cardioprotective activity of Biofield Energy Treated test item, Dulbecco's Modified Eagle Medium (DMEM) using rat cardiomyocytes (H9c2).

Methods: The test item (DMEM) was divided into three parts, first part received one-time Biofield Energy Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group.

Results: Cell viability of the test samples by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay showed 89.03% and 98.49% in the BT-I and BT-II groups, respectively suggested a nontoxic and safe in nature of the tested test item. The BT-I group showed 16.01% restoration of cell viability. The level of lactate dehydrogenase (LDH) was significantly inhibited by 50.37% and 49.35% in the BT-I and BT-II groups, respectively compared to the untreated DMEM group. Moreover, percent protection of creatine kinase-myocardial band (CK-MB) by 49.48% and 59.79% in the BT-I and BT-II groups, respectively, compared to the untreated DMEM group. Reactive oxygen species (ROS) level in terms of mean fluorescence unit (FU) was reduced by 6.64% in the BT-I group than untreated DMEM. Besides, BT-I and BT-II groups significantly increased the level of % apoptotic cells by 63.16% and 97.37% (p≤0.05), respectively than untreated DMEM.

Conclusion: Allover, results envisaged that Biofield Treatment significantly improved different cardiac parameters. Thus, Biofield Energy Treatment (The Trivedi Effect®) could be utilized as a cardio-protectant against several cardiac disorders such as coronary artery disease, heart attack, arrhythmias, heart failure, congenital heart disease, cardiomyopathy, etc.

References

1. CDC, NCHS. Underlying Cause of Death 1999-2013 on CDC WONDER Online Database, released 2015. Data are from the Multiple Cause of Death Files, 1999-2013, as compiled from data provided by the 57 vital statistics jurisdictions through the Vital Statistics Cooperative Program. Accessed Feb. 3, 2015.

2. Atlas of Heart Disease and stroke, WHO, Sept. 2004.

3. Benjamin EJ, Virani SS, Callaway CW, Chang AR, Cheng S et al. (2018) Heart disease and stroke statistics - 2018 update: A report from the American Heart Association Circulation. DOI: 10.1161/CIR.0000000000000558.

4. Diet, Nutrition and the Prevention of Chronic Diseases. WHO, Geneva, 2003.

5. Rakesh S, Arunporn I (2017) Herbal supplements or herbs in heart disease: Herbiceutical formulation, clinical trials, futuristic developments. J Cardiol Cardiovasc Ther.3(1),555603.

6. Peter AK, Bjerke MA, Leinwand LA (2016) Biology of the cardiac myocyte in heart disease. Drubin DG, ed. Molecular Biology of the Cell.27(14),2149-2160.

7. Kuznetsov AV, Javadov S, Sickinger S, Frotschnig S, Grimm M (2015) H9c2 and HL-1 cells demonstrate distinct features of energy metabolism, mitochondrial function and sensitivity to hypoxia-reoxygenation. Biochimica et Biophysica Acta.1853(2),276-284.

8. Duthie SJ, Melvin WT, Burke MD (1994) Bromobenzene detoxification in the human liver-derived HepG2 cell line. Xenobiotica.24,265-279.

9. Lutgendorf SK, Mullen-Houser E, Russell D, Degeest K, Jacobson G et al. (2010) Preservation of immune function in cervical cancer patients during chemoradiation using a novel integrative approach. Brain Behav Immun.24,1231-1240.

10. Ironson G, Field T, Scafidi F, Hashimoto M, Kumar M et al. (1996) Massage therapy is associated with enhancement of the immune system's cytotoxic capacity. Int J Neurosci.84,205-217.

11. Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R et al. (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med.4,58-66.

12. Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med.8,703-717.

13. Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol.4,141.

14. Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther.7, 253-257.

15. Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep.63,22-28,31.

16. Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage.4,161.

17. Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O et al. (2015) Effect of biofield energy treatment on physical and structural properties of calcium carbide and praseodymium oxide. International Journal of Materials Science and Applications.4,390-395.

18. Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S et al. (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom.3,129.

19. Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of biofield modality on viral load of Hepatitis B and C viruses. J Antivir Antiretrovir.7,83-88.

20. Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis.5,189.

21. Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC et al. (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences.3, 245-250.

22. Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC et al. (2015) Evaluation of biochemical marker – Glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience.3,243-248.

23. Trivedi MK, Branton A, Trivedi D, Nayak G, Plikerd WD et al. (2017) A Systematic study of the biofield energy healing treatment on physicochemical, thermal, structural, and behavioral properties of magnesium gluconate. International Journal of Bioorganic Chemistry.2,135-145.

24. Parulkar VR, Trivedi MK, Branton A, Trivedi D, Nayak G et al. (2018) Improved metabolism of vitamin d3 in human osteoblasts cells after biofield energy healing treatment. American Journal of Laboratory Medicine.3,11-19.

25. Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol.7,203-206.

26. Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci.1,1-9.

27. Branton A, Jana S (2017) The influence of energy of consciousness healing treatment on low bioavailable resveratrol in male Sprague Dawley rats. International Journal of Clinical and Developmental Anatomy.3,9-15.

28. Branton A, Jana S (2017) The use of novel and unique biofield energy healing treatment for the improvement of poorly bioavailable compound, berberine in male Sprague Dawley rats. American Journal of Clinical and Experimental Medicine.5,138-144.

29. Branton A, Jana S (2017) Effect of The biofield energy healing treatment on the pharmacokinetics of 25-hydroxyvitamin D3 [25(OH)D3] in rats after a single oral dose of vitamin D3. American Journal of Pharmacology and Phytotherapy.2,11-18.

30. Parulkar VR, Trivedi MK, Branton A, Trivedi D, Nayak G et al. (2017) The use of consciousness energy healing based herbomineral formulation for skin anti-aging strategies. Journal of Food and Nutrition Sciences.5,96-106.

31. Singh J, Trivedi MK, Branton A, Trivedi D, Nayak G et al. (2017) Consciousness energy healing treatment based herbomineral formulation: A safe and effective approach for skin health. American Journal of Pharmacology and Phytotherapy.2,1-10.

32. Anagnos D, Trivedi K, Branton A, Trivedi D, Nayak G et al. (2018) Influence of biofield treated vitamin D3 on proliferation, differentiation, and maturation of bone-related parameters in MG-63 cell-line. International Journal of Biomedical Engineering and Clinical Science.4,6-14.

33. Lee AC, Trivedi K, Branton A, Trivedi D, Nayak G et al. (2018) The potential benefits of biofield energy treated vitamin D3 on bone mineralization in human bone osteosarcoma cells (MG-63). International Journal of Nutrition and Food Sciences.7,30-38.

34. Stutheit ME, Trivedi K, Branton A, Trivedi D, Nayak G et al. (2018) Biofield energy treated vitamin D3: Therapeutic implication on bone health using osteoblasts cells. American Journal of Life Sciences.6,13-21.

35. Branton A, Jana S (2019) Improved metabolic cardiac biomarkers activity using rat cardiomyocytes cell line (H9c2) against biofield energy treated test sample. J Cardiol.3(1),000137.

36. Alía M, Ramos S, Mateos R, Bravo L, Goya L (2005) Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J Biochem Mol Toxicol.19,119-128.

37. Vargas-Mendoza N, Madrigal-Santillán E, Morales-González A, Esquivel-Soto J, Esquivel-Chirino C et al. (2014) Hepatoprotective effect of silymarin. World J Hepatol.6,144-149.

38. Webb C, Twedt D (2008) Oxidative stress and liver disease. Vet Clin North Am Small Anim Pract.38, 125-135.

39. Sha Li, Hor-Yue Tan, Ning Wang, Zhang-Jin Zhang, Lixing Lao et al. (2015) The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci.16,26087-26124.

40. Cheresh P, Kim SJ, Tulasiram S, Kamp DW (2013) Oxidative stress and pulmonary fibrosis. Biochim Biophys Acta.1832,1028-1040.

41. Lu LY, Ou N, Lu QB (2013) Antioxidant induces DNA damage, cell death and mutagenicity in human lung and skin normal cells. Sci Rep.3,3169.

42. Burgner JW, Ray WJ (1984) On the origin of the lactate dehydrogenase induced rate effect. Biochemistry.23,3636-3648.

43. Valvona CJ, Fillmore HL, Nunn PB, Pilkington GJ (2015) The regulation and function of lactate dehydrogenase A: Therapeutic potential in brain tumor. Brain Pathol.26,3-17.

44. Kopperschläger G, Kirchberger J (1996) Methods for the separation of lactate dehydrogenases and clinical significance of the enzyme. J Chromatogr B Biomed Appl.684(1-2),25-49.

45. Arram EO, Fathy A, Abdelsamad AA, Elmasry EI (2014) Value of cardiac biomarkers in patients with acute pulmonary embolism. Egypt J Chest Dis Tuberc.63(1),247-252.

46. Wang J, Wang F (2017) The detection value of CK-MB, Myo and cTnIin in patients with AMI and HF. Biomed Res.28(19),8533-8536.

47. Guzy PM (1977) Creatine phosphokinase-MB (CPK-MB) and the diagnosis of myocardial infarction. West J Med.127(6),455-460.

48. He F, Zuo L (2015) Redox roles of reactive oxygen species in cardiovascular diseases. Miller FJ, ed. Int J Mol Sci.16(11),27770-27780.

49. Halliwell B (1984) Oxygen radicals: A commonsense look at their nature and medical importance. Med Biol.62(2),71-77.

50. Zuo L, Best TM, Roberts WJ, Diaz PT, Wagner PD (2015) Characterization of reactive oxygen species in diaphragm. Acta Physiol (Oxf).213(3),700-710.

51. Zuo L, Shiah A, Roberts WJ, Chien MT, Wagner PD et al. (2013) Low Po₂ conditions induce reactive oxygen species formation during contractions in single skeletal muscle fibers. Am J Physiol Regul Integr Comp Physiol.304(11),R1009-R1016.

52. Tarpley MM, Wink DA, Grisham MB (2004) Methods for detection of reactive metabolites of oxygen and nitrogen: In vitro and in vivo considerations. Am J Physiol Regul Integr Comp Physiol.286,R431-R444.

53. Bennett MR (2002) Apoptosis in the cardiovascular system. Heart.87(5),480-487.

54. Saraste A, Voipio-Pulkki LM, Parvinen M, Pulkki K (1997) Apoptosis in the heart. N Engl J Med.336,1025-1026.

55. Kang PM, Izumo S (2003) Apoptosis in heart: Basic mechanisms and implications in cardiovascular diseases. Trends Mol Med.9,177-182.

56. Elliott MR, Ravichandran KS (2010) Clearance of apoptotic cells: Implications in health and disease. J Cell Biol.189(7),1059-1070.

Cite this work

Researchers should cite this work as follows:

  • Mahendra Kumar Trivedi, Snehasis Jana (2019) In vitro Assessment of the Biofield Treated Test Item on Cardiac Function Using Rat Cardiomyocytes Cell Line (H9c2) via Multiparametric Analysis. Journal Of Hypertension And Cardiology - 2(4):1-12.
     

  • Mahendra Kumar Trivedi; Snehasis Jana (2019), "In vitro Assessment of the Biofield Treated Test Item on Cardiac Function Using Rat Cardiomyocytes Cell Line (H9c2) via Multiparametric Analysis," https://diagrid.org/resources/1927.

    BibTex | EndNote

Tags